Search results for "Cell specification"

showing 4 items of 4 documents

Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo

1994

Two homeobox-containing genes that belong to different homeodomain classes have been isolated from a sea urchin geonomic library. One, PlHbox11, is the sea urchin homologue of the human and mouse Hox B3 gene, the other, PlHbox12, shows about 55% identity with paired class genes. Expression profile analysis of the two sea urchin Hbox genes suggests that they play different roles during embryogenesis. In fact, PlHbox11 transcripts are rare and are detected only in the pluteus larva and in the Aristotle's lantern and intestine of the adult. The PlHbox12 gene is, on the contrary, transiently expressed in the very early embryo already at the four cell stage; it accumulates at the 64 cell stage a…

Blastomeresanimal structuresMolecular Sequence DataSettore BIO/11 - Biologia MolecolarePlant ScienceBiologyMicebiology.animalGeneticsAnimalsHumansAmino Acid SequenceRNA MessengerCloning MolecularHox geneGeneSea urchinRegulation of gene expressionSequence Homology Amino AcidEmbryogenesisGenes HomeoboxGene Expression Regulation DevelopmentalEmbryocell specificationGeneral MedicineBlastomereSequence Analysis DNAMolecular biologyhomeodomainInsect ScienceSea Urchinsembryonic structuresHomeoboxAnimal Science and Zoologyembryogenesispaired
researchProduct

In vivo fate mapping with SCL regulatory elements identifies progenitors for primitive and definitive hematopoiesis in mice.

2009

10 páginas, 6 figuras.-- et al.

Definitive hematopoiesisEmbryologyMyeloidPopulationConditional mouse modelIn vivo linage and fate tracingEmbryonic DevelopmentStem cell leukemia geneBiology03 medical and health sciencesMice0302 clinical medicineFate mappinghemic and lymphatic diseasesProto-Oncogene ProteinsCRE systemmedicineBasic Helix-Loop-Helix Transcription FactorsAnimalsCell LineageMesodermal blood cell specificationGene Knock-In TechniquesProgenitor celleducationGeneTetracycline systemT-Cell Acute Lymphocytic Leukemia Protein 1Primitive hematopoiesis030304 developmental biology0303 health scienceseducation.field_of_studyMicroscopy ConfocalStem CellsEmbryoFlow CytometryCell biologyHematopoiesisGastrulationHaematopoiesismedicine.anatomical_structureBlood cell precursors030220 oncology & carcinogenesisImmunologyIn vivo lineage markingDevelopmental BiologyMechanisms of development
researchProduct

Role of en and novel interactions between msh, ind, and vnd in dorsoventral patterning of the Drosophila brain and ventral nerve cord

2010

AbstractSubdivision of the neuroectoderm into discrete gene expression domains is essential for the correct specification of neural stem cells (neuroblasts) during central nervous system development. Here, we extend our knowledge on dorsoventral (DV) patterning of the Drosophila brain and uncover novel genetic interactions that control expression of the evolutionary conserved homeobox genes ventral nervous system defective (vnd), intermediate neuroblasts defective (ind), and muscle segment homeobox (msh). We show that cross-repression between Ind and Msh stabilizes the border between intermediate and dorsal tritocerebrum and deutocerebrum, and that both transcription factors are competent t…

Neuroectodermal regionalizationNervous systemengrailedEmbryo Nonmammaliananimal structuresCentral nervous systemDorsoventral (DV) patterningBiologyNeuroblastAnterior Horn CellsmedicineAnimalsDrosophila Proteinsmsh/MsxMolecular Biologyind/GsxBody PatterningHomeodomain ProteinsVentral nerve cord (VNC)GeneticsNeuroectodermBrainvnd/Nkx2Cell BiologyBrain developmentengrailedNeural stem cellCell biologymedicine.anatomical_structureVentral nerve cordHomeoboxDrosophilaTranscription FactorsStem cell specificationDevelopmental BiologyDevelopmental Biology
researchProduct

Spatially restricted expression of PlOtp, a Paracentrotus lividus Orthopedia-related homeobox gene, is correlated with oral ectodermal patterning and…

1999

ABSTRACT Several homeobox genes are expressed in the sea urchin embryo but their roles in development have yet to be elucidated. Of particular interest are homologues of homeobox genes that in mouse and Drosophila are involved in patterning the developing central nervous system (CNS). Here, we report the cloning of an orthopedia (Otp)-related gene from Paracentrotus lividus, PlOtp. Otp is a single copy zygotic gene that presents a unique and highly restricted expression pattern. Transcripts were first detected at the mid-gastrula stage in two pairs of oral ectoderm cells located in a ventrolateral position, overlying primary mesenchyme cell (PMC) clusters. Increases in both transcript abund…

animal structuresDNA ComplementaryStomodeumBody PatterningPolarity in embryogenesisCell specificationCleavage Stage OvumMolecular Sequence DataGene DosageGene ExpressionSettore BIO/11 - Biologia MolecolareEctodermNerve Tissue ProteinsParacentrotus lividusGene expressionEctodermmedicineAnimalsDrosophila ProteinsAmino Acid SequenceCloning MolecularMolecular BiologyBody PatterningGeneticsHomeodomain ProteinsbiologyBase SequenceGenes HomeoboxOrthopediaSequence Analysis DNAbiology.organism_classificationCell biologymedicine.anatomical_structureEctopic expressionParacentrotus lividusSea UrchinsSpiculogenesisSettore BIO/03 - Botanica Ambientale E Applicataembryonic structuresHomeoboxEctopic expressionDevelopmental Biology
researchProduct